I would’ve already posted on how to make Treated cow urine (TCU) and what it is, but now I have done this experiment. What I did is made TCU with the aerator. I took two drums of TCU side by side number ‘1’ with the air pump and number ‘2’ without. I found that ‘1’ was not as powerful and thick as ‘2’. I am not 100% sure that the experiment went fully as planned but from the result that I got, I could clearly say that ‘1’ was not even close to the rich and thick number ‘2’. Well from this it is sensible to conclude that TCU might have anaerobic bacteria!! As I said this is not tested it is my guess. Hope it makes sense!